水稻淡绿叶突变体HM14的遗传分析与基因定位

doi:10.3969/j.issn.1001-7216.2013.06.004

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (6): 585-590.DOI: 10.3969/j.issn.1001-7216.2013.06.004

水稻淡绿叶突变体HM14的遗传分析与基因定位

施勇烽^＃,魏彦林^＃,奉保华,王惠梅,徐霞,黄奇娜,吕向光,张晓波,吴建利^*

中国水稻研究所水稻生物学国家重点实验室/国家水稻改良中心, 杭州 310006；

收稿日期:2013-06-27 修回日期:2013-07-15 出版日期:2013-11-10 发布日期:2013-11-10
通讯作者: 吴建利*
基金资助:
国家863计划资助项目（2012AA101102，2011AA10A101）；水稻生物学国家重点实验室自主课题资助项目（ZZKT200801）。

Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice

SHI Yongfeng^＃, WEI Yanlin^＃, FENG Baohua, WANG Huimei, XU Xia, HUANG Qina, LV Xiangguang, ZHANG Xiaobo, WU Jianli^*

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; #These authors contributed equally to this paper;

Received:2013-06-27 Revised:2013-07-15 Online:2013-11-10 Published:2013-11-10
Contact: WU Jianli*

摘要/Abstract

摘要： 淡绿叶突变体HM14是通过EMS诱变籼稻IR64获得的，整个生育期叶色均保持淡绿。与野生型相比，突变体除了株高显著降低外，其他农艺性状未发生显著改变。光合色素含量和光合指数测定结果表明，突变体的叶绿素a、b含量较野生型IR64明显下降，突变体的净光合速率显著降低，但蒸腾速率明显上升，光合作用受到显著影响。遗传分析结果表明，突变体淡绿叶性状受一对隐性核基因控制。构建突变体HM14与Moroberekan的F2分离群体作为基因定位群体，将该淡绿叶基因pglHM14定位在第11染色体短臂RM1812和RM26092两个标记之间大约299 kb区间内。

关键词: 水稻, 淡绿叶突变体, 叶绿素含量, 基因定位

Abstract: The palegreen leaf mutant HM14 was isolated from an EMSinduced IR64 mutant bank. The mutant showed a stable palegreen leaf phenotype throughout the whole life cycle. The plant height of HM14 decreased significantly while other agronomic traits were largely similar to the wildtype IR64. The chlorophyll content of HM14 reduced notably at tillering stage when compared with IR64. The net photosynthetic rate of HM14 was significantly lower than the wildtype IR64 at filling stage while the transpiration rate was raised significantly. Genetic analysis showed that the pale green leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using both the mutant and wildtype DNA pools. The gene pglHM14 was finally delimited to a region of 299 kb between SSR markers RM1812 and RM26092 on the short arm of chromosome 11.

Key words: rice, pale green leaf mutant, chlorophyll content, gene mapping

中图分类号:

施勇烽＃,魏彦林＃,奉保华,王惠梅,徐霞,黄奇娜,吕向光,张晓波,吴建利*. 水稻淡绿叶突变体HM14的遗传分析与基因定位[J]. 中国水稻科学, 2013, 27(6): 585-590.

SHI Yongfeng＃, WEI Yanlin＃, FENG Baohua, WANG Huimei, XU Xia, HUANG Qina, LV Xiangguang, ZHANG Xiaobo, WU Jianli*. Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice[J]. Chinese Journal of Rice Science, 2013, 27(6): 585-590.

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水稻淡绿叶突变体HM14的遗传分析与基因定位

Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice

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