水稻产量相关农艺性状杂种优势位点的定位

doi:10.3969/j.issn.1001-7216.2013.06.002

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (6): 569-576.DOI: 10.3969/j.issn.1001-7216.2013.06.002

水稻产量相关农艺性状杂种优势位点的定位

王智权^1，2 ,江玲¹,尹长斌³,王晓玲²,雷建国²,肖宇龙² ,刘喜¹ ,刘世家¹,陈亮明¹ ,余传元² ,万建民^1，3，*

¹南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心, 南京 210095; ²江西省农业科学院水稻研究所/水稻国家工程实验室, 南昌 330200; ³中国农业科学院作物科学研究所, 北京 100081;

收稿日期:2013-02-28 修回日期:2013-08-19 出版日期:2013-11-10 发布日期:2013-11-10
通讯作者: 万建民1，3，*
基金资助:
国家自然科学（地区）基金资助项目（31260356）；国家863计划资助项目(2009AA101101)；江西省自然科学青年基金资助项目（20114BAB214008）；江西省农业科学院博士启动基金资助项目（2011CBS003）。

QTL Mapping of Heterotic Loci of Yieldrelated Traits in Rice

WANG Zhiquan ^1,2, JIANG Ling ¹, YIN Changbin ³, WANG Xiaoling ², LEI Jianguo ², XIAO Yulong ², LIU Xi ¹, LIU Shijia ¹, CHEN Liangming ¹, YU Chuanyuan ², WAN Jianmin ^1，3，*

¹Research Center of Jiangsu Plant Gene Engineering/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; ²Jiangxi Academy of Agricultural Sciences/ National Engineering Laboratory of Rice, Nanchang 330200, China; ³Crop Science Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

Received:2013-02-28 Revised:2013-08-19 Online:2013-11-10 Published:2013-11-10
Contact: WAN Jianmin1，3，*

摘要/Abstract

摘要： 利用以籼稻IR24为受体亲本、粳稻Asominori为供体亲本的66个染色体片段置换系，分别与受体亲本IR24杂交构建1套对应的F1群体，研究杂种优势位点。以中亲优势值作为杂种优势QTL检测的基准表型值，结合新构建的基因型图谱，利用QTL IciMapping软件的逐步回归和极大似然估计结合的方法，检测杂种优势QTL。结果表明，各置换系对应的F1群体，在4个环境（2007年南京、2007年南昌、2008年南京、2008年南昌）下，共检测到53个与产量构成性状相关的杂种优势位点。其中只发现1个杂种优势位点能在多个环境重复检测到，即来自第1染色体与标记RM488紧密连锁的位点。该位点在杂合状态下增加F1的株高。22个位点在杂合状态下具有增效作用，占总位点数的41.51%；LOD值变幅为3.06~7.25，贡献率变幅为3.74%~18.5%。31个位点在杂合状态下具有减效作用，LOD值变幅为3.07~9.70，贡献率变幅为0.45%~3078%，这些具有减效作用的位点主要控制单株产量、每穗实粒数和结实率等性状，与籼粳杂种不育基因密切相关。

关键词: 水稻, 染色体片段置换系, 产量构成性状, 杂种优势位点

Abstract: A total of 66 chromosome segment substitution lines, derived from a cross between indica inbred line IR24 (as the recurrent parent) and japonica inbred line Asominori (as the donor parent), were used to investigate the heterotic loci in indica/ japonica intersubspecific rice hybrids. Each line was crossed with the background parent IR24, and the heterosis of F1 hybrids was estimated by comparing the F1 with its two parental lines. Field experiments were carried out across four different environments (2007, 2008 in Nanjing; 2007, 2008 in Nanchang) to evaluate yield and yieldrelated traits in the 66 lines and their 66 corresponding F1 hybrids. Quantitative trait loci (QTL) analyses were conducted using a likelihood ratio test based on the stepwise regression. QTL were detected by statistical software QTL IciMapping using midparental heterosis as basic phenotypic data through a new SSR map. As a result, 53 heterotic loci of yieldcomponent traits were identified with significant effects in different environments. Only one heterotic locus linked closely with marker RM488 on chromosome 1 was detected repeatedly in more than one environment,which might improve the plant height in F1 derivatives. Of all the heterotic loci, 22 (41.51%) showed positive effects, with LOD values ranging from 3.06 to 7.25, explaining 3.74% to 18.5% of the phenotypic variance respetively; 31 loci showed negative effects, with LOD values ranging from 3.07 to 9.70, explaining 0.45% to 30.78% of the phenotypic variance, respectively; Those loci with negative effects mainly affected the traits of grain weight per plant, no. of grains per panicle and seedsetting rate, which were closely related to those genes of hybrid sterility between indica and japonica rice.

Key words: rice, chromosome segments substitution lines, yield component traits, heterosis loci

中图分类号:

王智权1，2 ,江玲1,尹长斌3,王晓玲2,雷建国2,肖宇龙2 ,刘喜1 ,刘世家1 ,陈亮明1 ,余传元2 ,万建民1，3，*. 水稻产量相关农艺性状杂种优势位点的定位[J]. 中国水稻科学, 2013, 27(6): 569-576.

WANG Zhiquan1,2, JIANG Ling1, YIN Changbin3, WANG Xiaoling2, LEI Jianguo2, XIAO Yulong2, LIU Xi1, LIU Shijia1, CHEN Liangming1, YU Chuanyuan2, WAN Jianmin1，3，*. QTL Mapping of Heterotic Loci of Yieldrelated Traits in Rice[J]. Chinese Journal of Rice Science, 2013, 27(6): 569-576.

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水稻产量相关农艺性状杂种优势位点的定位

QTL Mapping of Heterotic Loci of Yieldrelated Traits in Rice

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