中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (4): 434-439.DOI: 10.3969/j.issn.1001-7216.2013.04.014

• 实验技术 • 上一篇    下一篇

基于均匀设计优化的大螟实用饲料配方及继代饲养

戴长庚1，2 ,李凯龙2 ,王立峰1 ,谭显胜1  ,胡阳2 ,袁哲明1,* ,傅强1,2,*   

  1. 1湖南农业大学 生物安全科学技术学院，长沙410128；2中国水稻研究所 水稻生物学国家重点实验室，杭州310006；
  • 收稿日期:2013-01-17 修回日期:2013-04-17 出版日期:2013-07-10 发布日期:2013-07-10
  • 通讯作者: 袁哲明1,* ,傅强1,2,*
  • 基金资助:

    转基因生物新品种培育重大专项(2011ZX08012004)；国家科技支撑计划资助项目(2012BAD19B03)。

An Oligidic Diet for Sesamia inferens Optimized by Uniform Design and Successive Rearing

DAI  Changgeng 1,2， LI  Kailong 2， WANG  Lifeng 1， TAN Xiansheng  1， HU  Yang 2， YUAN  Zheming 1，*， FU Qiang 1,2,*   

  1. 1 College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128，  China;  2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006， China;
  • Received:2013-01-17 Revised:2013-04-17 Online:2013-07-10 Published:2013-07-10
  • Contact: YUAN Zheming1，*， FU Qiang1,2,*

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摘要： 通过均匀设计对大螟人工饲料配方进行了优化，获得的实用饲料优化配方如下：琼脂3 g，大豆粉8 g，茭白粉11 g，酵母16 g，蔗糖6 g，稻茎粉3 g，玉米粉11 g，麦芽粉5 g，干酪素7 g，维生素C 0.8 g，山梨酸0.64 g，金霉素0.54 g，胆固醇004 g，无机盐1.5 g，蒸馏水200 g。该配方对大螟已连续饲养超过10个世代，优于以往报道的饲料。该配方饲养的大螟第1代初孵化幼虫发育至蛹的比例（化蛹率87.5%）较原配方（CK，40.8%）提高46.7个百分点。从第1代到第10代的连续饲养过程中，大螟的化蛹率、羽化率、卵孵化率和单雌产卵量等指标没有明显退化趋势，平均分别为42.5%~87.5%、79.7%~958%、90.0~94.9%和95~252粒/雌。第10代除雄幼虫历期显著较长外，其余各指标均与第1代无显著差异。

关键词: 大螟, 实用人工饲料, 均匀设计, 继代饲养

Abstract: An oligidic diet for larvae rearing of the pink stem borer (PSB), Sesamia inferens (Walker), was optimized by using the uniform design. The formulation of the optimized diet was as follows: agar powder 3 g, soybean powder 8 g, water bamboo powder 11 g, yeast powder 16 g, sucrose 6 g, rice powder 3 g, corn powder 11 g, wheat germ powder 5 g, casein 7 g, vitamin C 0.8 g, sorbic acid 0.64 g, aureomycin 0.54 g, cholesterol 0.04 g, Wesson’s salt 15 g, and distilled water 200 g. PSB had been reared on the diet for more than ten successive genenrations, much more than that previously reported. The rate of the 1st instar PSB larvae that developed to pupae on the optimized diet reached 87.5%, significantly higher than that on  the benchmark diet. As for the ten generations’ succesive rearing, the average of pupation rate, emergence rate, egg hatching rate and fecundity of PSB on the optimized diet was 42.5%-87.5%, 79.7%-95.8%, 90.0%-94.9% and 95-252 eggs per female, respectively. There was no significant difference between the 10th genenation and the 1st of PSB reared on the diet, except the significantly longer male larval stage.

Key words: Sesamia inferens, oligidic diet, uniform design, successive rearing

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