中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (3): 329-334.DOI: 10.3969/j.issn.10017216.2013.03.015

• 研究简报 • 上一篇    

分子标记辅助选择创制抗白背飞虱水稻恢复系

张建福1 ,曾大力2 ,朱永生1 ,谢鸿光1 ,蔡秋华1 ,连玲1 ,吴方喜1 ,罗曦1 ,王颖姮1 ,郑轶1 ,谢华安1，*   

  1. 1福建省农业科学院 水稻研究所/农业部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/杂交水稻国家重点实验室华南基地/水稻国家工程实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地，福建 福州 350003；2中国水稻研究所 水稻生物学国家重点实验室,  浙江 杭州 310006；
  • 收稿日期:2012-07-18 修回日期:2013-03-06 出版日期:2013-05-10 发布日期:2013-05-10
  • 通讯作者: 谢华安1，*
  • 基金资助:

    国家863计划资助项目（2011AA10A101）；国家转基因农作物新品种培育重大专项（2009ZX08001003B）； 福建省农业科学院青年创新基金资助项目（2010QC9）。

Breeding of Rice Restore Lines with Whitebacked Planthopper Resistance by MarkerAssisted  Selection

ZHANG Jianfu1 , ZENG Dali2 , ZHU Yongsheng1, XIE Hongguang1, CAI Qiuhua1, LIAN Ling1, WU Fangxi1, LUO Xi1, WANG Yingheng1, ZHENG Yi1, XIE Huaan1, *   

  1. 1 Rice Research Institute, Fujian Academy of Agricultural Sciences/Key Laboratory of  Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture,  China/Fuzhou Branch, National Rice Improvement Center of China/The Breedind Base of National Key Laboratory of Hybrid Rice in South China/National Rice Engineering Laboratory of China/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology, Fuzhou 350003, China; 2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 
  • Received:2012-07-18 Revised:2013-03-06 Online:2013-05-10 Published:2013-05-10
  • Contact: XIE Huaan1, *

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摘要： 将分子标记辅助选择技术与传统育种的杂交、回交方法及白背飞虱频发区大田自然诱发抗虫鉴定相结合，创制了米质优、配合力好且具有水稻白背飞虱拒食抗性主效QTL位点qSI4 的新恢复系材料3份(R38、R46和R58)。室内抗性鉴定结果表明，新创制的恢复系材料的白背飞虱抗性与抗源春江06的抗性水平相当，具有较强的白背飞虱抗性。这些新恢复系通过与不同遗传背景的不育系进行杂交配组，经过田间测产和室内米质鉴定、评比，筛选出一批集抗性好、米质优、产量高等优良性状于一体的杂交水稻新组合。

关键词: 杂交水稻, 恢复系, 白背飞虱, 分子标记辅助选择, 育种

Abstract: The whitebacked planthopper (WBPH, Sogatella furcifera) affects rice production and threatens food security in the world. It is very important to breed hybrid rice restorer lines with WPBH  resistance for rice production. The WBPH resistance locus  qSI4 from GL77 and GL84 were introduced into the susceptible restorer lines by molecular markerassisted selection (MAS), traditional breeding methods and WBPHresistance identification in multiple fields. Three improved lines of R38, R46 and R58, conferring WBPH resistance and with good agronomic characteristics were obtained by molecular marker test for the target genes and different parental genetic background analysis. The results indicated that the WBPH resistance of these new restorer lines with qSI4 was not significantly different to that   of Chunjiang 06. New hybrid rice combinations with WBPH resistance, high quality and high yield were bred.

Key words: hybrid rice, restorer line, whitebacked planthopper, markerassisted selection, breeding

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