钙信号试剂对水稻种子根负向光性生长的影响

doi:10.3969/j.issn.10017216.2013.03.006

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (3): 265-272.DOI: 10.3969/j.issn.10017216.2013.03.006

钙信号试剂对水稻种子根负向光性生长的影响

陈娟¹,莫亿伟^2,3，* ,胥华伟⁴

¹黄冈师范学院化学与生命科学学院/经济林木种质改良与资源综合利用湖北省重点实验室，湖北黄冈 438000； ²中国热带农业科学院南亚热带作物研究所广东湛江 524091； ³绍兴文理学院生命科学学院，浙江绍兴 312000； ⁴河南科技大学农学院，河南洛阳 471003；

收稿日期:2012-07-16 修回日期:2012-12-12 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 莫亿伟2,3，* ,
基金资助:
国家自然科学基金资助项目（31071353）; 经济林木种质改良与资源综合利用湖北省重点实验室（黄冈师范学院）开放基金资助项目(2013000503)。

Effect of Calcium Signaling Reagent on Negative Phototropism of Rice Seminal Roots

CHEN Juan ¹, MO Yiwei ^2，3，* , XU Huawei⁴

¹Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of chemistry and life science, Huanggang Normal University, Huanggang 438000, China; ²South Subtropical Crop Research Institute, Chinese Academy of tropical Agricultural Science, Zhenjiang 524091, China; ³ College of Life Science, Shaoxing University, Shaoxing 312000, China; ⁴ College of Agriculture, Henan University of Science and Technology, Luoyang 471003, China;

Received:2012-07-16 Revised:2012-12-12 Online:2013-05-10 Published:2013-05-10
Contact: MO Yiwei2，3，*,

摘要/Abstract

摘要： 为了探讨水稻根负向光性形成与钙信号传导的关系，以水稻种子根为材料，用不同浓度的钙信号试剂\[氯化钙、钙通道有机阻断剂异搏定、钙通道无机阻断剂氯化镧、钙调素抑制剂氯丙嗪（CPZ）以及生长素极性运输抑制剂NPA\]处理水稻种子根，以1/10 Hoagland培养液为对照，并用100~200 μmol/(m2·s)单侧光照射24 h。实验结果表明，适量的CaCl2使水稻种子根负向光性增强并使其生长加快，负向光性增强是由于外源Ca2+进一步促进生长素从向光侧向背光侧运输引起的，与生长速率加快无关；同时，随着钙通道有机阻断剂、无机阻断剂、钙调素抑制剂以及生长素极性运输抑制剂浓度的上升，水稻根的负向光性及生长均受显著抑制，当异搏定的浓度大于100 μmol/L，LaCl3的浓度大于12.5 μmol/L，负向光性消失，种子根的生长也受到严重的影响；而60 μmol/L钙调素抑制剂氯丙嗪、6 μmol/L 生长素极性运输抑制剂NPA也使得根负向光性消失，根的生长几乎停止；向LaCl3溶液、异搏定溶液以及NPA溶液中添加100 μmol/L CaCl2可使负向光性及生长速率得到不同程度的恢复，表明钙离子作为第二信使系统，与生长素相互作用，参与光信号调控水稻根生长和负向光性形成。

关键词: 水稻, 根, 负向光性, 钙信号, 生长素极性运输, 钙离子通道阻断剂

Abstract: In order to investigate the relationship between calcium signaling and negative phototropism of rice roots, different concentration of calcium signaling reagent such as CaCl2, organic calcium channel blocker(verapamil), inorganic calcium channel blocker(LaCl3), calcineurin inhibitor chloropromaize（CPZ), auxin polar transport inhibitor(NPA) were used to treat rice seminal roots which were unilaterally illuminated at an intensity of 100-200 μmol/(m2·s) for 24 h. Results showed that negative phototropism curvature and growth rate of rice roots were enhanced by a proper dose of CaCl2. Further experiments testified that it is because more IAA was transported from irradiated side to shaded side by exogenous Ca2+ that negative phototropism curvature was enhanced rather than increased growth rate. Simultaneously, negative phototropism curvature and growth rate of rice roots were inhibited by inhibitors above, and the inhibiting effects were enhanced as the rising concentration of inhibitors. Roots would stop growing and negative phototropism characteristic would disappear when the concentration reached a certain degree, such as 100 μmol/L verapamil, 12.5 μmol/L LaCl3, 60 μmol/L CPZ and 6 μmol/L NPA. 100 μmol/L CaCl2 relieved the inhibition degree of LaCl3, verapamil and NPA. The results indicated that calcium ion plays an important role as a second messenger in the process of light signaling regulating rice roots growth and negative phototropism.

Key words: rice, root, negative phototropism, calcium signaling, polar auxin transport, calcium channel blockers

中图分类号:

陈娟1 ,莫亿伟2,3，* ,胥华伟4. 钙信号试剂对水稻种子根负向光性生长的影响[J]. 中国水稻科学, 2013, 27(3): 265-272.

CHEN Juan1, MO Yiwei2，3，*, XU Huawei4. Effect of Calcium Signaling Reagent on Negative Phototropism of Rice Seminal Roots[J]. Chinese Journal of Rice Science, 2013, 27(3): 265-272.

参考文献

\[1\]Pepper A E, Seong K M, Hebst S M, et al. shl， a new set of Arabidopsis mutants with exaggerated developmental responses to available red，farred and bluelight. Plant Physiol, 2001, 127(1): 295304.

\[2\]MartinTryon E L, Harmer S L. XAP5 CIRCADIAN TIMEKEEPER coordinates light signals for proper timing of photomorphogenesis and the circadian clock in Arabidopsis. Plant Cell, 2008, 20(5): 12441259.

\[3\]Okada K, Yoshiro S. Mutational analysis of root gravitropism and phototropism of Arabidopsis thaliana seedings. Aust J Plant Physiol, 1992, 19(4): 439 448.

\[4\]Briggs W R, Beck C F, Cashmore A R, et al. The phototrophin family of photoreceptors. Plant Cell, 2001, 13(5): 993997.

\[5\]Wang Z, Mo Y, Qian S, et al. Negative phototropism of rice root and its influencing factors. Sci China: Ser C, 2002, 45(5): 485496.

\[6\]Mo Y, Wang Z, Qian S, et al. Effects of IAA on the negative phototropism of rice root. Chin J Rice Sci, 2004, 11(3): 125128.

\[7\]Vatha S, Zhao L, Sack F D. Internaction of root gravitropism and phototropism in Arabidopsis wildtype and starchless mutants. Plant Physiol, 2000, 122(2): 453461.

\[8\]White P J. Calcium channels in higher plants. Biochem Biophys Acta, 2000, 1465(1/2): 171189.

\[9\]Wang Y F, Fan L M, Zhang W Z, et al. Ca2+permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments. Plant Physiol, 2004, 136(4): 38923904.

\[10\]Hepler P K.Calcium: A central regulator of plant growth and development. Plant Cell, 2005, 17(8): 21422155.

\[11\]Volotovski I D. Role of calcium ions in photosignaling processes in a plant cell. Biophysics, 2011, 56(5): 778788.

\[12\]Bjrkman T, Leopold A C. Effect of inhibitors of auxin transport and of calmodulin on a gravisensingdependent current in maize roots. Plant Physiol, 1987, 84(3): 847850.

\[13\]Toyota M, Furuichi T, Tatsumi H, et al. Cytoplasmic calcium increases in response to changes in the gravity vector in hypocotyls and petioles of arabidopsis seedlings. Plant Physiol, 2008, 146(2): 505514.

\[14\]Lee J S, Mulkley T J, Evans M L. Reversible loss of gravitropic sensitivity in maize roots after tip application of calcium chelators.Science, 1983, 220(4604): 13751376.

\[15\]Daye S, Biro R L, Roux S J.Inhibition of gravitropism in oat coleoptiles by the calcium chelator, ethyleneglycolbis(βaminoethyl ether)N′tetraacetic acid. Physiol Planta, 1984, 61(3): 449454.

\[16\]Lee J S, Mulkley T J, Evans M L.Gravity induced polar transport of calcium across root tips of maize. Plant Physiol, 1983, 73(4): 874876.

\[17\]Bjrkman T, Cleland R. The role of extracellular free Ca2+ gradients in gravitropic signalling in maize roots. Planta, 1991, 185(3): 379384.

\[18\]Gehring C A, Irving H R, Parish R W. Effects of auxin and abscisic acid on cytosolic calcium and pH in plant cells. Proc Natl Acad Sci USA, 1990, 87(24): 96459649.

\[19\]Monshausen G B, Miller N D, Murphy A S, et al. Dynamics of auxindependent Ca2+ and pH signaling in root growth revealed by integrating highresolution imaging with automated computer visionbased analysis. Plant J, 2011, 65(2): 309318.

\[20\]Felle H.Auxin causes oscillations of cytosolic free calcium and pH in Zea mays coleoptiles. Planta, 1988, 174(4): 495499.

\[21\]Hepler P K, Wayne R O. Calcium and plant development. Annu Rev Plant Physiol, 1985, 36: 397439.

\[22\]莫亿伟, 王忠, 钱善勤, 等. 生长素在水稻根负向光性反应中的作用. 中国水稻科学, 2004, 18(3): 245248.

\[23\]Rosen E, Chen R, Masson P H. Root gravitropism: A complex response to a simple stimulus? Trends Plant Sci, 1999, 4(10): 407412.

\[24\]马力耕，周君莉，安宜，等. 钙在红光调节的尾穗苋苋红素合成中的作用.科学通报, 1995, 40(10): 944946.

\[25\]Pineros M, Tester M. Calcium channels in higher plant cells: Selectivity, regulation and pharmacology. J Exp Bot, 1997, 48: 551577.

\[26\]Feijó J A, Malhó R, Obermeyer G. Ion dynamics and its possible role during in vitro pollen germination and tube growth. Protoplasma, 1995, 187(1/4): 155167.

\[27\]Kimura M, Kagawa T. Phototropin and lightsignaling in phototropism. Curr Opin Plant Biol, 2006, 9(5): 503508.

\[28\]Hasenstein K H, Evans M L. Calcium dependence of rapid auxin action in maize roots. Plant Physiol, 1986, 81: 439443.

\[29\]Evans, M L. Gravitropism: Interaction of sensitivity modulation and effector redistribution. Plant Physiol, 1991, 95(1): 15.

\[30\]Yamagami M, Haga K, Napier R M, et al. Two distinct signaling pathways participate in auxininduced swelling of pea epidermal protoplasts. Plant Physiol, 2004, 134(2): 735747.

\[31\]Raghothamak G, Mizrahi Y, Poovaiahb W. Effect of calmodulin antagonists on auxininduced elongation. Plant Physiol, 1985, 79(1): 2833.

\[32\]Plieth C, Trewavas A J.Reorientation of seedlings in the earth′s gravitational field induces cytosolic calcium transients. Plant Physiol, 2002, 129(2): 786796.

\[33\]Bush D S.Calcium Regulation in plant cells and its role in signaling. Annu Rev Plant Biol, 1995, 46: 95122.

\[34\]Perera I Y, Hung C Y, Brady S,et al. A universal role for inositol 1,4,5trisphosphatemediated signaling in plant gravitropism. Plant Physiol, 2006, 140(2): 746760.

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钙信号试剂对水稻种子根负向光性生长的影响

Effect of Calcium Signaling Reagent on Negative Phototropism of Rice Seminal Roots

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