中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (3): 247-258.DOI: 10.3969/j.issn.10017216.2013.03.004

• 研究报告 • 上一篇    下一篇

粳稻8个异交相关性状及其中亲优势的QTL定位与遗传分析

张红1 ,江建华1,2 ,刘晓丽1 ,吉家曾1 ,牛付安1 ,张有富1,3 ,洪德林1,*   

  1. 1南京农业大学 作物遗传与种质创新国家重点实验室, 江苏 南京210095; 2安徽省农业科学院 作物研究所,  安徽 合肥  230031; 3河西学院 农学系， 甘肃 张掖 734000;
  • 收稿日期:2012-08-09 修回日期:2012-09-22 出版日期:2013-05-10 发布日期:2013-05-10
  • 通讯作者: 洪德林1,*
  • 基金资助:

    国家863计划资助项目(2010AA101301)。

QTL Mapping and Genetic Analysis of Eight Outcrossingrelated Traits and Its Midparental Heterosis in japonica Rice

ZHANG Hong 1, JIANG Jianhua 1, 2, LIU Xiaoli 1, JI Jiazeng 1, NIU Fuan 1, ZHANGYoufu 1,3, HONG Delin 1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Crops, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 3 Department of Agronomy,Hexi College,Zhangye 734000, China;
  • Received:2012-08-09 Revised:2012-09-22 Online:2013-05-10 Published:2013-05-10
  • Contact: HONG Delin1,*

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摘要： 为探明控制粳稻异交相关性状及其中亲优势的基因作用类型，利用秀堡RIL群体及其2个回交(BCF1)群体对穗抽出度、剑叶长、剑叶角度、穗剑高度差、倒2叶长、倒2叶角度、穗与倒2叶（穗二）高度差和倒1节间长8个异交相关性状及其中亲优势进行QTL定位。3个群体中共检测到45个显著的主效QTL（MQTL），单个MQTL的贡献率变幅为1.5%～803%。73.3%的MQTL表现为加性效应，4.5%的MQTL表现为部分或完全显性效应，22.2%的MQTL表现为超显性效应。3个群体中共检测到82对显著的双基因上位性QTL（EQTL）。RIL群体中检测到43对EQTL，单对EQTL的贡献率变幅为1.0%～7.0%，平均2.7%。在以秀水79为父本、与秀堡RIL群体回交的后代（XSBCF1群体）中检测到16对EQTL，其中利用BCF1表型值检测到11对EQTL，单对EQTL的贡献率变幅为11.2%～36.8%，平均21.0%；利用中亲优势值检测到6对EQTL，单对EQTL的贡献率变幅为33.1%～76.8%，平均55.0%。在以C堡为父本、与秀堡RIL群体回交的后代（CBBCF1群体）中检测到23对EQTL，其中利用BCF1表型值检测到16对EQTL，单对EQTL的贡献率变幅为6.2%～60.0%，平均24.0%；利用中亲优势值检测到7对EQTL，单对EQTL的贡献率变幅为21.3%～44.4%，平均31.0%。上述结果表明，粳稻异交相关性状是由多位点控制的，基因对性状本身的作用类型以加性效应为主；粳稻异交相关性状中亲优势主要遗传基础为超显性效应和上位性效应。

关键词: 粳稻, 异交性状, 主效QTL, 上位性QTL

Abstract: To understand the types of gene action controlling outcrossrelated traits and its midparental heterosis, QTL mapping of eight outcrossingrelated traits including panicle exsertion degree (PED), flag leaf length (FLL), flag leaf angle (FLA), vertical distance between panicle tip and flag leaf tip (DPFT), second leaf length (SLL), second leaf angle (SLA), vertical distance between panicle tip and second leaf tip (DPST) and length of the first internode (LFI) from the top was conducted using 254 Xiushui 79/C Bao recombinant inbred lines (RILs) and two backcross hybrid populations derived from these RILs. 45 maineffect QTLs (MQTL) were identified in the three populations. The percentage of phenotypic variance explained by each QTL ranged from 1.5% to 80.3%. Among them, 73.3% QTL showed an additive effect, 4.5% a partialtocomplete dominant effect, and 22.2% an overdominant effect. Eightytwo pairs of digenic epistatic QTL (EQTL) were detected in the 3 populations,of which 43 pairs were detected in RIL population, and the percentage of phenotypic variance explained by each pair of QTL ranged from 1.0% to 7.0%, with an average 2.7%. Sixteen pairs of EQTL were detected in XSBCF1 population. Among them, 11 pairs were detected by using XSBCF1 phenotypic value, and the percentage of phenotypic variance explained by each EQTL ranged from 11.2% to 36.8%, with an average 21.0%. Six pairs of EQTL were detected by using midparental heterosis value (HMP), and the percentage of phenotypic variance explained by each EQTL ranged from 33.1% to 76.8%, with an average 55.0%. Twentythree pairs of EQTL were detected in CBBCF1 population. Among them, 16 pairs of EQTL were detected by using BCF1 phenotypic value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 6.2% to 60.0%, with an average 24.0%. Seven pairs of EQTL were detected by using the midparental heterosis value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 21.3% to 44.4%, with an average 31.0%. These results showed that outcrossingrelated traits themselves were mainly controlled by multiple loci with additive effects, and the primary genetic basis of midparent heterosis for outcrossingrelated traits was overdominant and epistatic effect in  japonica  rice.

Key words: japonica rice, outcrossrelated trait, maineffect QTL, epistatic QTL

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