抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

doi:10.3969/j.issn.10017216.2013.03.001

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (3): 223-230.DOI: 10.3969/j.issn.10017216.2013.03.001

• 研究报告 • 下一篇

抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

李磊^1,# ,薛芗^1,# ,左示敏¹,陈宗祥¹,张亚芳¹,李前前¹,朱俊凯²,马玉银³,潘学彪^1,* ,潘存红 ^2,*

¹扬州大学农学院, 江苏扬州 225009; ²江苏里下河地区农业科学研究所, 江苏扬州 225007; ³扬州职业大学生化工程系, 江苏扬州 225009;

收稿日期:2012-12-13 修回日期:2013-01-24 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 潘学彪1,*,潘存红2,*
基金资助:
国家自然科学基金资助项目（31201180）；江苏省自然科学基金资助项目（BK2008223；BK2012690）。

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

LI Lei ^1,# , XUE Xiang ^1,# , ZUO Shimin ¹ , CHEN Zongxiang ¹ , ZHANG Yafang ¹, LI Qianqian¹ , ZHU Junkai ² , MA Yuyin ³ , PAN Xuebiao ^1,* , PAN Cunhong ^2,*

¹ Agricultural College, Yangzhou University, Yangzhou 225009, China; ² Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China; ³ Department of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou 225009, China; #These authors contribute equally to this paper;

Received:2012-12-13 Revised:2013-01-24 Online:2013-05-10 Published:2013-05-10
Contact: PAN Xuebiao1,*, PAN Cunhong2,*

摘要/Abstract

摘要： AGO蛋白是RNA诱导沉默复合体的核心元件，在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11~12外显子的片段，构建了两个干涉载体OsAGO1aI1E和OsAGO1aI2E。利用农杆菌介导法转化水稻品种日本晴，抑制OsAGO1a基因的表达，借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明, 抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。

关键词: 水稻, OsAGO1a, 极性发育, 卷叶, 农艺性状

Abstract: Argonaute (AGO) protein is the core effectors in RNAinduced silencing complexes (RISCs), playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs: OsAGO1aI1E and OsAGO1aI2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacteriummediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits.

Key words: rice, OsAGO1a, polarity development, rolled leaf, agronomic traits

中图分类号:

李磊1,#,薛芗1,#,左示敏1,陈宗祥1,张亚芳1,李前前1,朱俊凯2,马玉银3,潘学彪1,*,潘存红2,*. 抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲[J]. 中国水稻科学, 2013, 27(3): 223-230.

LI Lei1,#, XUE Xiang1,#, ZUO Shimin1, CHEN Zongxiang1, ZHANG Yafang1, LI Qianqian1, ZHU Junkai2, MA Yuyin3, PAN Xuebiao1,*, PAN Cunhong2,* . Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice[J]. Chinese Journal of Rice Science, 2013, 27(3): 223-230.

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抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

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