云南地方稻种抗稻瘟病基因Pita和Pib的鉴定

doi:10.3969/j.issn.10017216.2012.05.012

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (5): 592-598.DOI: 10.3969/j.issn.10017216.2012.05.012

云南地方稻种抗稻瘟病基因Pita和Pib的鉴定

李进斌¹,王甜²,许明辉^2,*

¹ 云南省农业科学院农业环境资源研究所，云南昆明 650205； ² 云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创新重点实验室，云南昆明 650223；

收稿日期:2011-07-29 修回日期:2012-05-22 出版日期:2012-09-10 发布日期:2012-09-10
通讯作者: 许明辉2,*
基金资助:
国家自然科学基金资助项目（31160355）；云南省自然科学基金资助项目（2010ZC173）；云南省教育厅基金资助项目（09Y0198）。

Identification of Pita and Pib Genes for Rice Blast Resistance of Rice Landraces from Yunnan Province

LI Jinbin ¹, WANG Tian ², XU Minghui ^2,*

¹ Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences， Kunming 650205,China; ² Key Laboratory of Biotechnology Research of Yunnan Province/ Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences/Key Laboratory of Southwest Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223,China;

Received:2011-07-29 Revised:2012-05-22 Online:2012-09-10 Published:2012-09-10
Contact: XU Minghui2,*

摘要/Abstract

摘要： 根据抗病等位基因Pita、Pib与pita、pib感病等位基因在DNA序列上的差异，合成Pita引物YL155/YL87和YL183/YL87，Pib基因引物PibdomF/PibdomR和Lys145F/Lys145R。利用这两套分子标记检测了176份云南地方稻种抗性基因Pita和Pib的分布。结果表明，供试材料中72份含有Pita基因，占40.9%，其中99份粳稻中有28份含有Pita基因，占28.3%；77份籼稻中有44份含有该基因，占57.1%；抗性基因Pita在粳稻和籼稻品种中的分布频率存在明显差异，籼稻高于粳稻；Pita基因主要分布于玉溪、保山、临沧、思茅等地区。而176份供试材料中仅有33份含有Pib基因，占18.8%，其中99份粳稻中有18份含有该基因，占18.2%；77份籼稻中有15份含有该基因，占19.5%；Pib基因主要分布在滇西部的怒江、保山和昭通等地区。分别选取具有代表性的18及17份材料在温室进行Pita和Pib基因抗性鉴定，结果与分子鉴定结果基本相符。

关键词: 地方稻种, 稻瘟病, 分子标记

Abstract: The existence of the two genes of Pita and Pib in 176 rice landraces from Yunnan Province were rapidly determined using the dominant DNA markers of Pita/pita and Pib/pib alleles(YL155/YL18 and YL183/YL87 for the former, PibdomF/Pib dom R and Lys14SF/Lys145R for the latter designed according to their difference in sequence). The results showed that Pita was found in 72 among 176 landraces with the frequency of 40.9%. Among them, Pita was found in 44 among 77 indica and 28 among 99 japonica cultivars, the frequency in indica and japonica were 57.1% and 28.3%, respectively. The distribution frequency of the resistance gene Pita in the japonica and indica varieties significantly differed, the frequency was higher in indica than in japonica cultivars. While Pib was found in 33 among 176 landraces with the frequency of 18.8%. Among them, Pib was found in 15 indica and 18 japonica cultivars, the frequency in indica and japonica were 19.5% and 18.2%, respectively. Pita was mainly distributed in Yuxi, Baoshan, Lincang and Simao prefectures, while Pib was mainly distributed in Nujiang, Baoshan and Zhaotong prefectures of Yunnan Province. The results of pathogenic assays with the rice blast fungus in greenhouse were generally consistent with the results of molecular identification.

Key words: landrace, rice blast, molecular marker

中图分类号:

李进斌1,王甜2,许明辉2,*. 云南地方稻种抗稻瘟病基因Pita和Pib的鉴定[J]. 中国水稻科学, 2012, 26(5): 592-598.

LI Jinbin1, WANG Tian2, XU Minghui2,*. Identification of Pita and Pib Genes for Rice Blast Resistance of Rice Landraces from Yunnan Province[J]. Chinese Journal of Rice Science, 2012, 26(5): 592-598.

参考文献

\[1\]Zhai C, Lin F, Dong Z Q, et al. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol, 2010： 114.

\[2\]Hayashi N, Inoue H, Kato T, et al. Durable panicle blastresistance gene Pb1 encodes an atypical CCNBSLRR protein and was generated by acquiring a promoter through local genome duplication. Plant J, 2010, 64(3): 498510.

\[3\]Okuyama Y, Kanzaki H, Abe A, et al. A multifaceted genomics approach allows the isolation of the rice Piablast resistance gene consisting of two adjacent NBSLRR protein genes. Plant J, 2011, 66(3):467479.

\[4\]Yuan B, Zhai C, Wang W J, et al. The Pikp resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CCNBSLRR genes. Theor Appl Genet, 2011, 122（5）：10171028.

\[5\]Takahashi A, Hayashi N, Miyao A, et al. Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposontagging. BMC Plant Biol, 2010, 10:175.

\[6\]Wang Z, Yano M, Yomanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine rich repeat class of plant resistance genes. Plant J, 1999, 19: 5564.

\[7\]Bryan G, Wu K, Farrall L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pita. Plant Cell, 2000, 12: 20332045.

\[8\]Wang Z, Jia Y, Rutger J, et al. Rapid survey for presence of a blast resistance gene Pita in rice cultivars using the dominant DNA markers derived from portions of the Pita gene. Plant Breeding, 2007, 126: 3642.

\[9\]刘洋，徐培洲，张红宇，等. 水稻抗稻瘟病Pib基因的分子标记辅助选择与应用. 中国农业科学, 2008, 41(1): 914.

\[10\]王忠华，贾育林，吴殿星，等. 水稻抗稻瘟病基因Pita的分子标记辅助选择. 作物学报, 2004, 30(12): 12591265.

\[11\]许明辉，郑明慧，刘彦中. 烟草品种RAPD分子标记多态性与品种鉴定. 种子, 1998（5）： 2325.

\[12\]Jia Y, Wang Z, Singh P. Development of dominant rice blast Pita resistance gene markers. Crop Sci, 2002, 42: 21452149.

\[13\]Jia Y, Wang Z, Robert G, et al. Rice Pita gene confers resistance to the major pathotypes of the rice blast fungus in the United States. Phytopathology, 2004, 94: 296301.

\[14\]Concetta A, Anna M, Marco A, et al. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes. Crop sci, 2004, 44: 17901798.

\[15\]时克，雷财林，程治军，等. 稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布. 植物遗传资源学报, 2009, 10(1) : 2126.

\[16\]Mohan M, Nair S, Bhagwat A, et al. Genome mapping molecular marker and markerassisted selection in crop plants. Mol Breeding, 1997, 3: 87103.

\[17\]Li M F, Zhou K D. Rice Biotechnological Breeding. Beijing: China Agricultural Science and Technology Press, 2001, 239269.

\[18\]Kiyosawa S. Gene analysis of blast resistance in exotic varieties of rice. Jpn Agric Res Quart, 1971, 6: 18.

\[19\]Fukuta Y. A differential system for rice blast disease. JIRCAS Newsl, 2002, 32: 6.

\[20\]王忠华， Redus MARC，贾育林. 水稻稻瘟病基因Pita共显性分子标记的建立. 中国水稻科学, 2005, 19(6): 483488.

\[21\]李进斌，李成云，陈艳，等. 二十二个抗稻瘟病基因在云南的利用价值评价. 植物保护学报, 2005, 6(2): 113119.

\[22\]Jia Y, Bryan G T, Farrall L, et al. Natural variation at the Pita rice blast resistance locus. Phytopathology, 2003, 93(11): 14521459.

\[23\]Wang X, Jia Y, Shu Q Y, et al. Haplotype diversity at the Pita locus in cultivated rice and its wild relatives. Phytopathology, 2008, 98: 13051311.

\[24\]Huang C L, Hwang S Y, Chiang Y C, et al. Molecular evolution of the Pita gene resistant to rice blast in wild rice (Oryza rufipogon). Genetics, 2008, 179: 15271538.

[1]	许丹洁, 林巧霞, 李正康, 庄小倩, 凌宇, 赖美玲, 陈晓婷, 鲁国东. OsOPR10正调控水稻对稻瘟病和白叶枯病的抗性[J]. 中国水稻科学, 2024, 38(4): 364-374.
[2]	童琪, 王春燕, 阙亚伟, 肖宇, 王政逸. 稻瘟病菌热激蛋白(HSP)40编码基因MoMHF6的鉴定及功能研究[J]. 中国水稻科学, 2023, 37(6): 563-576.
[3]	冯爱卿, 汪聪颖, 苏菁, 封金奇, 陈凯玲, 林晓鹏, 陈炳, 梁美玲, 杨健源, 朱小源, 陈深. 水稻细菌性条斑病抗性新品系的创制及其农艺性状分析[J]. 中国水稻科学, 2023, 37(6): 587-596.
[4]	陈明亮, 熊文涛, 沈雨民, 熊焕金, 罗世友, 吴小燕, 胡兰香, 肖叶青. 广谱抗稻瘟病水稻保持系赣香B的抗性遗传解析[J]. 中国水稻科学, 2023, 37(5): 470-477.
[5]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[6]	王雨, 孙全翌, 杜海波, 许志文, 吴科霆, 尹力, 冯志明, 胡珂鸣, 陈宗祥, 左示敏. 利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性[J]. 中国水稻科学, 2023, 37(2): 125-132.
[7]	王石光, 陆展华, 刘维, 卢东柏, 王晓飞, 方志强, 巫浩翔, 何秀英. 应用CRISPR/Cas9技术与分子标记辅助选择创制广东丝苗米新种质[J]. 中国水稻科学, 2023, 37(1): 29-36.
[8]	陈涛, 赵庆勇, 朱镇, 赵凌, 姚姝, 周丽慧, 赵春芳, 张亚东, 王才林. 利用分子标记辅助选择培育优良食味、低谷蛋白香粳稻新品系[J]. 中国水稻科学, 2023, 37(1): 55-65.
[9]	周永林, 申小磊, 周立帅, 林巧霞, 王朝露, 陈静, 冯慧捷, 张振文, 陈晓婷, 鲁国东. OsLOX10正调控水稻对稻瘟病和白叶枯病的抗性[J]. 中国水稻科学, 2022, 36(4): 348-356.
[10]	董铮, 王雅美, 黎用朝, 熊海波, 薛灿辉, 潘孝武, 刘文强, 魏秀彩, 李小湘. 基于MAGIC群体的水稻镉含量全基因组关联分析[J]. 中国水稻科学, 2022, 36(1): 35-42.
[11]	曹煜东, 肖湘谊, 叶乃忠, 丁晓雯, 易晓璇, 刘金灵, 肖应辉. 生长素调控因子OsGRF4协同调控水稻粒形和稻瘟病抗性[J]. 中国水稻科学, 2021, 35(6): 629-638.
[12]	刘树芳, 董丽英, 李迅东, 周伍民, 杨勤忠. 持有Pi9基因的水稻单基因系IRBL9-W对稻瘟病菌苗期和成株期抗性差异[J]. 中国水稻科学, 2021, 35(3): 303-310.
[13]	朱华珺, 周瑚, 任佐华, 刘二明. 枯草芽孢杆菌JN005胞外抗菌物质及对水稻叶瘟防治效果[J]. 中国水稻科学, 2020, 34(5): 470-478.
[14]	许赵蒙, 李利华, 高晓庆, 袁正杰, 李莘, 田旭丹, 王岚岚, 瞿绍洪. 转Pi9抗稻瘟病基因水稻株系的比较转录组分析[J]. 中国水稻科学, 2020, 34(3): 245-255.
[15]	孟峰, 张亚玲, 靳学慧. 黑龙江省稻瘟病菌无毒基因AVR-Pita及其同源基因的检测与分析[J]. 中国水稻科学, 2020, 34(2): 143-149.

云南地方稻种抗稻瘟病基因Pita和Pib的鉴定

Identification of Pita and Pib Genes for Rice Blast Resistance of Rice Landraces from Yunnan Province

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics