亚洲栽培稻程氏指数与SSR标记分类的比较分析

doi:10.3969/j.issn.10017216.2012.02.005

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (2): 165-172.DOI: 10.3969/j.issn.10017216.2012.02.005

亚洲栽培稻程氏指数与SSR标记分类的比较分析

王彩红,徐群,于萍,袁筱萍,余汉勇,王一平,汤圣祥,魏兴华*

中国水稻研究所水稻生物学国家重点实验室，浙江杭州 310006

收稿日期:2011-03-22 修回日期:2011-09-14 出版日期:2012-03-10 发布日期:2012-03-10
通讯作者: 魏兴华*
基金资助:
农业部物种资源保护专项基金资助项目。

Comparison of Cheng’s Index and SSR Markersbased Classification of Asian Cultivated Rice

WANG Caihong, XU Qun, YU Ping, YUAN Xiaoping, YU Hanyong, WANG Yiping, TANG Shengxiang, WEI Xinghua*

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;

Received:2011-03-22 Revised:2011-09-14 Online:2012-03-10 Published:2012-03-10
Contact: WEI Xinghua*

摘要/Abstract

摘要： 选取具有代表性的100份亚洲栽培稻品种，应用程氏指数和109个SSR分子标记对它们进行分类。结果表明，等位酶Ⅰ群（典型籼稻）和ⅥA群（温带粳稻）程氏指数和SSR标记亚结构特征均十分明显，拟合度较高（73.3%～100%）。Mantel检测揭示，程氏指数与SSR分子标记进行栽培稻分类存在明显的正相关，两种方法各个材料间遗传距离的相关达极显著水平（r＝0.466，P<0.01）。遗传结构和聚类分析表明，在籼粳亚种水平上，程氏指数和SSR标记分类结果趋于一致，但在亚种内亚结构分析中，程氏指数偏差较大，SSR标记具有更高的准确性。同时， SSR分析表明混合型材料多存在于Ⅱ群和Ⅵ群内部。

关键词: 亚洲栽培稻, 分类, 程氏指数, 简单重复序列

Abstract: A total of one hundred cultivated rice accessions were grouped based on Cheng’s index and SSR polymorphism. The results showed that the subpopulation features of allozyme groupⅠ (indica) and allozyme group ⅥA (temperate japonica) were obvious both classified by Cheng’s index and SSR marker, with goodness of fit ranging from 73.3% to 100%. Mantel test revealed that the Euclidean distance of Cheng’s index was significantly correlated with Nei’s genetic distance of 109 SSR loci (r=0.466, P<0.01). According to both modelbased structure and UPGMA cluster analysis, the classification results at subspecies level based on Cheng’s index coincided with that on SSR marker. Within subspecies, the subpopulations classified by Cheng’s phenotypic characters showed lower goodness of fit than SSR marker. In addition, allozyme group Ⅱ (aus, boro) and allozyme group Ⅵ (temperate japonica, tropical japonica) had more mixture types based on SSR locus analysis.

Key words: Oryza sativa L., classification, Cheng’s index, simple sequence repeat

中图分类号:

Q943
S511.03

王彩红,徐群,于萍,袁筱萍,余汉勇,王一平,汤圣祥,魏兴华*. 亚洲栽培稻程氏指数与SSR标记分类的比较分析[J]. 中国水稻科学, 2012, 26(2): 165-172.

WANG Caihong, XU Qun, YU Ping, YUAN Xiaoping, YU Hanyong, WANG Yiping, TANG Shengxiang, WEI Xinghua*. Comparison of Cheng’s Index and SSR Markersbased Classification of Asian Cultivated Rice[J]. Chinese Journal of Rice Science, 2012, 26(2): 165-172.

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亚洲栽培稻程氏指数与SSR标记分类的比较分析

Comparison of Cheng’s Index and SSR Markersbased Classification of Asian Cultivated Rice

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