中国水稻科学

中国水稻科学 ›› 2011, Vol. 25 ›› Issue (6): 587-593.DOI: 10.3969/j.issn.10017216.2011.06.004

• 研究报告 • 上一篇    下一篇

一个水稻新型叶色突变体的形态结构与遗传定位

李育红1,2王宝和2 戴正元2 李爱宏2刘广青2 左示敏1张洪熙1,2,* 潘学彪1，*   

  1. 1扬州大学 江苏省作物遗传生理重点实验室， 江苏 扬州225009; 2 江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心， 江苏 扬州 225007；
  • 收稿日期:2010-12-17 修回日期:2011-01-10 出版日期:2011-11-10 发布日期:2011-11-10
  • 通讯作者: 张洪熙1,2,* 潘学彪1，*
  • 基金资助:

    江苏省自然科学基金资助项目（BK2010294）；江苏省(教育部)植物功能基因组学重点实验室开放课题（K10001）。

Morphological Structure and Genetic Mapping of a New Leaf Color Mutant  in Rice

LI Yuhong 1,2, WANG Baohe 2, DAI Zhengyuan 2 , LI Aihong 2 , LIU Guangqing 2 , ZUO Shimin 1, ZHANG Hongxi 1,2,* , PAN Xuebiao 1，*   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China;  2 Lixiahe Region Agricultural esearch Institute of Jiangsu Province, Nanjing Subcenter of National Rice Improvement Center, Yangzhou 225007, China;
  • Received:2010-12-17 Revised:2011-01-10 Online:2011-11-10 Published:2011-11-10
  • Contact: ZHANG Hongxi1,2,*, PAN Xuebiao1，*

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被引次数

27

摘要： 所用水稻叶色突变体为自然突变，并命名为白淮稻7号，其叶色表型为绿白绿，且突变表型只有在移栽等因素引起的机械损伤信号胁迫下才会产生。研究结果表明，叶色转白前，突变体生长态势、叶色、叶绿素a含量和叶绿体超显微结构与野生型差异不大；叶色转白后，突变体总叶绿素、叶绿素a、叶绿素b和类胡萝卜素含量都显著低于野生型和叶色转白前，而叶绿体中的类囊体逐渐降解，基粒片层减少、基粒数量明显减少，且在成熟后突变体叶色黄化、植株变矮小。遗传分析表明，突变性状由1对隐性核基因控制。以该突变体与江西1587的F2群体为定位群体，将突变基因定位于水稻第11染色体分子标记L59.27和L64.811之间大约740.5 kb的区间内。认为该突变基因是一个新的水稻叶色突变基因，暂命名为GWGL。

关键词: 水稻, 叶色突变体, 形态结构, 遗传分析, 基因定位

Abstract: A natural leaf color mutant  gwgl in rice, named Baihuaidao 7,  showed  a typical phenotype of greenwhitegreen leaf at different stages. The mutant character only arose under   signal stress induced by mechanical  damage  such as transplantation.  The   leaf color, chlorophyll a  content and ultrastructure of chloroplast of the  mutant were almost the same with the wild typle at  the seedling stage. After the leaf changed white, total chlorophyll, chlorophyll a, chlorophyll b and carotenoid  contents of the  mutant were evidently decreased, the thylakoid  of the mutant were  gradually degraded, and the chloroplast grana lamella and grana were decreased. At maturity, the plant of the mutant was etiolated  and dwarfed. The genetic analysis indicated that the leaf mutant was controlled by a recessive nuclear gene. Genetic mapping of the mutant gene was conducted by using  the F2 mapping population of Baihuaidao 7/Jiangxi 1587. The results suggested that the mutant gene was mapped on rice chromosome 11 between InDel markers L59.27 and L64.811. The physical distances between the  two InDel markers were about 740.5 kb. The mutant gene was considered to be a new leaf gene in rice and designated tentatively as GWGL，which laid a good foundation for finemapping, cloning and functional analysis of the mutant genes. 

Key words: Oryza sativa, leaf color mutant, morphological structure, genetic analysis, gene mapping

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