中国水稻科学 ›› 2011, Vol. 25 ›› Issue (6): 567-574.DOI: 10.3969/j.issn.10017216.2011.06.001

• 研究报告 •    下一篇

水稻丙酮酸脱羧酶基因OsPDC3功能的初步研究

陈波,韩斌*   

  1. 中国科学院 上海生命科学研究院 植物生理生态研究所/中国科学院 国家基因研究中心, 上海  200233
  • 收稿日期:2011-05-04 修回日期:2011-05-25 出版日期:2011-11-10 发布日期:2011-11-10
  • 通讯作者: 韩斌*
  • 基金资助:

    国家863计划资助项目(2006AA10A102); 国家自然科学基金资助项目(30771159)

Primary Function Analysis of a  Pyruvate Decarboxylase Gene, OsPDC3, in Rice

CHEN  Bo,  HAN  Bin*   

  1. National Center for Gene Research/Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, 
    Chinese Academy of Sciences, Shanghai  200233, China
  • Received:2011-05-04 Revised:2011-05-25 Online:2011-11-10 Published:2011-11-10
  • Contact: HAN Bin*

摘要: 植物花粉中存在着活跃的有氧发酵过程。丙酮酸脱羧酶(PDC)作为发酵途径中的关键酶,参与花粉中的能量和物质代谢,在花粉萌发过程中起重要作用。通过反向遗传学的方法对水稻丙酮酸脱羧酶基因OsPDC3的功能进行了初步分析。OsPDC3是一个单外显子基因,编码蛋白与另外4个水稻PDC蛋白间的序列一致性达到77%~82%。表达模式分析显示OsPDC3在花粉中特异表达,GUS组织染色进一步证实其启动子具有花粉特异表达活性。超量表达OsPDC3会使转基因植株叶片中的PDC酶活性上升,表明OsPDC3在体内具有活性功能,能够参与花粉内的生理活动。反义抑制下调了OsPDC3在花粉中的表达水平,但对花粉的离体萌发率没有产生影响,推测这是由于PDC基因间的冗余造成的。

关键词: 水稻, 丙酮酸脱羧酶, OsPDC3, 花粉

Abstract: There are high aerobic fermentation rates in plant pollen. As  a key enzyme of ethanolic fermentation pathway, pyruvate decarboxylase (PDC) is involved in  energy production and biosynthesis in pollen,  playing  an important role in the pollen germination process.OsPDC3  gene function was investigated by the reverse genetics methods. The gene is  a single exon gene encoding a protein that shares      77%-82%   sequence identity  with other rice PDC proteins. Expression pattern analysis showed  that the OsPDC3  specifically expressed in pollen, and  the pollen specific expression activity of the promoter of  OsPDC3 was confirmed by GUS staining. Overexpression of OsPDC3 in transgenic plants improved  the    activity of PDC in leaves, indicating functional enzyme activity of OsPDC  in vivo.  Antisense suppression downregulated  the expression of OsPDC3 in pollen, but the pollen germination rate in vitro  remained  unchangable, which might attribute to  the redundancy among the PDC genes.

Key words: rice, pyruvate decarboxylase, OsPDC3, pollen

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