水稻中一个与OsHSP90互作的新基因特性分析

doi:10.3969/j.issn.1001-7216.2009.04.02

中国水稻科学

水稻中一个与OsHSP90互作的新基因特性分析

马炳田^{1, 2，#},万佳^3,#,张琦^2,#,江斌¹,徐永菊¹,李冬梅¹,徐正君^1，2，*

¹四川农业大学水稻研究所，四川温江 611130； ²四川农业大学西南作物基因资源与遗传改良教育部重点实验室，四川雅安 625014； ³四川省农业厅植物检疫站，四川成都 610041； ﹟共同第一作者；*通讯联系人， E-mail: mywildrice@yahoo.com.cn

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-10 发布日期:2009-07-10

Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

MA Bingtian^{1, 2,﹟}, WAN Jia^3,﹟, ZHANG Qi^2,﹟, JIANG Bin¹, XU Yongju¹, LI Dongmei¹, XU Zhengjun^{1, 2, *}

¹Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; ²Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China; ³ Plant Quarantine Station of Sichuan Agriculture Department, Chengdu 610041, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: mywildrice@yahoo.com.cn

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-10 Published:2009-07-10

摘要/Abstract

摘要： OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统，在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因，命名为OsHSPBP。序列分析表明，该基因全长1321 bp，编码一个具有244个氨基酸残基的多肽，推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域，185-211位氨基酸残基构成CCT_2基序，与其他植物中的同源蛋白相似性介于30.66%～70.40%。经分析，该基因启动子区域存在6种不同的逆境反应顺式作用元件。RTPCR半定量结果表明， OsHSP90与OsHSPBP在逆境胁迫下，均能被不同程度地诱导并呈现相似的表达趋势，说明两者可能与水稻逆境胁迫的应答和信号传递有关。

关键词: 水稻, 逆境胁迫, 诱导基因, 基因表达, 氨基酸序列, 酵母双杂交系统

Abstract: OsHSP90 is an abiotic stressinducible gene in rice. From the chilling and droughtinducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSP90 was identified by using yeast twohybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame (ORF) predicts a 244amino acid(aa) polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185-211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66%-70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to ciselements related to the abiotic stress response were identified using the PlantCARE software. Semiquantitative RTPCR showed that the OsHSP90 and OsHSPBP might be detected in the similar degree under different stresstreatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

Key words: rice, abiotic stress, inducible gene, gene expression, amino acid sequence, yeast twohybrid system

马炳田,万佳,张琦,江斌,徐永菊,李冬梅,徐正君. 水稻中一个与OsHSP90互作的新基因特性分析[J]. 中国水稻科学 2009,23(4): 342-348 . , DOI: 10.3969/j.issn.1001-7216.2009.04.02 .

MA Bingtian,﹟,WAN Jia,﹟,ZHANG Qi,﹟,JIANG Bin,XU Yongju,LI Dongmei,XU Zhengjun. Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice [J]. Chinese Journal of Rice Science 2009,23(4): 342-348 ., DOI: 10.3969/j.issn.1001-7216.2009.04.02 .

参考文献

[1]Kotak S, Larkindale J, Lee U, et al. Complexity of the heat stress response in plants. Curr Opin Plant Biol, 2007, 103: 310-316.

[2] Picard D. Heatshock protein 90, a chaperone for folding and regulation. Cell & Mol Life Sci, 2002, 59(10): 1640-1648.

[3]Zimmerman J L, Petri W, Meselson M. Accumulation of specific subsets of D. melanogaster heat shock mRNAs in normal development without heat shock. Cell, 1983, 32(4): 1161-1170.

[4]Krishna P, Sacco M, Cherutti J F, et al. Coldinduced accumulation of HSP90 transcripts in Brassica napus. Plant Physiol, 1995, 107(3): 915-923.

[5]Marrs K A, Casey E S, Capitant S A, et al. Characterization of two maize HSP90 heat shock protein genes: Expression during heat shock, embryogenesis, and pollen development. Dev Genet, 1993, 14(1): 27-41.

[6]Schmitz G, Schmidt M, Feierabend J. Characterization of a plastidspecific HSP90 homologue: Identification of a cDNA sequence, phylogenetic descendence and analysis of its mRNA and protein expression. Plant Mol Biol, 1996, 30(3): 479-492.

[7] Zhao R, Davey M, Hsu Y C, et al. Navigating the chaperone network: An integrative map of physical and genetic interactions mediated by the HSP90 chaperone. Cell, 2005, 120(5): 715-742.

[8]Stebbins C E, Russo A A, Schneider C, et al. Crystal structure of an HSP90geldanamycin complex: Targeting of a protein chaperone by an antitumor agent. Cell, 1997, 89(2): 239-250.

[9] Logemann J, Schell J, Willmitzer L. Improved method for the isolation of RNA from plant tissues. Anal Biochem, 1987, 163(1): 16-20.

[10]Wegele H, Muller L, Buchner J. HSP70 and HSP90: A relay team for protein folding. Rev Physiol Biochem Pharmacol, 2004, 151: 1-44.

[11]Frydman J. Folding of newly translated proteins in vivo: The role of molecular chaperones. Annu Rev Biochem, 2001, 70: 603-647.

[12]Liu D, Lu Z, Mao Z, et al. Enhanced thermotolerance of E. coli by expressed OsHSP90 from rice (Oryza sativa L.). Curr Microbiol, 2009, 58(2): 129-133.

[13]Thao N P, Chen L, Nakashima A, et al. RAR1 and HSP90 form a complex with Rac/Rop GTPase and function in innateimmune responses in rice. Plant Cell, 2007, 19(12): 4035-4045.

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水稻中一个与OsHSP90互作的新基因特性分析

Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

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