转中国对虾抗菌肽基因水稻抗白叶枯病效应初析

doi:10.3969/j.issn.1001-7216.2010.04.001

中国水稻科学

• 研究报告 • 下一篇

转中国对虾抗菌肽基因水稻抗白叶枯病效应初析

王维^1,# ,吴超1^,#,刘梅², 刘旭日¹,胡国成¹,斯华敏¹ ,孙宗修¹,刘文真^1,*,付亚萍^1,*

¹中国水稻研究所水稻生物学国家重点实验室，浙江杭州310006； ²中国科学院海洋研究所海洋生物技术研发中心，山东青岛 266071; # 共同第一作者； *通讯联系人， E-mail: lwzzju@163.com; cnrrifuyp@hotmail.com

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-10 发布日期:2010-07-10

Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight

WANG Wei^{1, #}, WU Chao^{1, #}, LIU Mei², LIU Xu-ri¹, HU Guo-cheng¹, SI Hua-min¹, SUN Zong-xiu¹, LIU Wen-zhen^1,*, FU Ya-ping^1,*

¹State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ² R & D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; ^#These authors contributed equally to this paper; *Corresponding author, Email: lwzzju@163.com; cnrrifuyp@hotmail.com

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-10 Published:2010-07-10

摘要/Abstract

摘要： 抗菌肽是一类具有抗菌活性的多肽类物质。利用农杆菌介导法将中国对虾抗菌肽基因np3 和 np5分别导入粳稻品种爱知旭中，PCR检测显示，T0代转np3基因和np5基因水稻植株的阳性率分别为36%和45%。 RTPCR检测T1代转基因水稻，结果表明抗菌肽基因在RNA水平上得到表达，而抗菌肽的表达对农艺性状未产生显著影响。用白叶枯病菌小种CR4分别对4个T1代转np3基因和np5基因株系进行接种，所有转基因株系均表现出比对照更高水平的抗性。为了验证抗菌肽基因的广谱抗性，进一步检测了np5转基因株系对3个菌株JS972、ZHE173和OS225 的抗性，发现中国对虾抗菌肽基因np5对白叶枯病菌可能具有较广谱的抗性。

关键词: 水稻, 抗菌肽, 白叶枯病, 抗性

Abstract: Antimicrobial peptides (AMP) is a polypeptide with antimicrobial activity. The antimicrobial peptide genes np3 and np5 from Chinese shrimp were integrated into Oryza sativa L. subsp. japonica. cv. Aichi ashahi by Agrobacterium mediated transformation system. PCR analysis showed that the positive ratios of np3 and np5 were 36% and 45% in T0 generation，respectively. RTPCR analysis showed that the antimicrobial peptide genes were expressed in T1 generation and there was no obvious difference in agronomic traits between transgenic plants and nontransgenic plants. Four np3 and np5 transgenic lines in T1 generation were inoculated with the strain CR4 of Xanthomonas oryzae pv. oryzae(Xoo), and the results indicated that all the transgenic lines had a significantly enhanced resistance to bacterial blight caused by the Xoo strain CR4. The np5 transgenic lines also conferred higher resistance to bacterial blight caused by the Xoo strains JS972, ZHE173 and OS225. It is suggested that transgenic lines with np5 gene might possess a broad spectrum resistance to rice bacterial blight.

Key words: rice, antimicrobial peptides, bacterial blight, resistance

王维,吴超,刘梅, 刘旭日,胡国成,斯华敏 ,孙宗修,刘文真付亚萍,. 转中国对虾抗菌肽基因水稻抗白叶枯病效应初析[J]. 中国水稻科学 2010,24(4): 335-340 . , DOI: 10.3969/j.issn.1001-7216.2010.04.001 .

WANG Wei,WU Chao,LIU Mei,LIU Xu-ri,HU Guo-cheng, SI Hua-min, SUN Zong-xiu, LIU Wen-zhen,FU Ya-ping. Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight[J]. Chinese Journal of Rice Science 2010,24(4): 335-340 ., DOI: 10.3969/j.issn.1001-7216.2010.04.001 .

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转中国对虾抗菌肽基因水稻抗白叶枯病效应初析

Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight

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