中国水稻科学

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转Pi-d2基因水稻对稻瘟病的抗性分析

陈德西1,2;陈学伟1;雷财林3; 马炳田1;王玉平1;李仕贵1,*   

  1. 1四川农业大学 水稻研究所, 四川 成都 611130; 2四川省农业科学院 植物保护研究所, 四川 成都 610066; 3中国农业科学院 作物科学研究所, 北京100081; *通讯联系人, E-mail: lishigui_sc@263.net
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-10 发布日期:2010-01-10

Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene

CHEN De-xi1,2, CHEN Xue-wei1, LEI Cai-lin3, MA Bing-tian1, WANG Yu-ping1, LI Shi-gui1,*   

  1. 1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; *Corresponding author, E-mail: lishigui_sc@263.net)

  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-10 Published:2010-01-10

摘要: 对由pCB63kb、pCB53kb和pZH01272kb三个不同表达载体转化的转稻瘟病抗性基因Pid2水稻株系进行稻瘟病抗性分析。结果表明,转Pid2基因水稻的9个高代株系对来自四川的39个稻瘟病菌株表现不同的抗性,抗病频率最高达917%;4个转基因早代纯合株系对来自中国农业科学院的58个菌株中的8148%以上菌株表现抗性,具有广谱抗性特点。稻瘟病菌粗毒素筛选结果表明,来自转基因植株的幼胚愈伤组织诱导率随培养基中粗毒素浓度提高而降低,粗毒素浓度达到40%时,幼胚愈伤诱导率为4933%,受体对照幼胚愈伤组织诱导率为5%。田间诱发条件下,转基因株系在大田的穗瘟发病率为0%~50%, 抗性较受体对照大幅度提高。

关键词: 转基因水稻, 抗病基因, 稻瘟病, 抗谱, 粗毒素

Abstract: Transgenic rice lines harboring Pid2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB53kb and pZH01272kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest diseaseresistance frequency was 917%. Four early homozygous transgenic lines of Pid2 gene exhibited resistance to more than 8148% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed widespectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 4933%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.

Key words: transgenic rice, resistance gene, rice blast, resistance spectrum, crude toxin