中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻幼苗耐缺氧能力的QTL分析

王洋1，2;郭媛1;洪德林1，*

  

  1. 1南京农业大学 作物遗传与种质创新国家重点实验室， 江苏 南京 210095； 2 黑龙江大学 农业资源与环境学院， 黑龙江 哈尔滨 150008； *通讯联系人， E-mail: delinhong@njau.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-10 发布日期:2010-01-10

QTL Analysis of the Anoxic Tolerance at the Seedling Stage in Rice

WANG Yang1，2 , GUO Yuan1, HONG Delin1，*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; *Corresponding author, E-mail: delinhong@njau.edu.cn

  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-10 Published:2010-01-10

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摘要： 利用粳稻品种秀水79与粳稻恢复系C堡及其衍生的247个重组自交系 (RIL)和粳稻Nipponbare与籼稻Kasalath及Nipponbare/Kasalath//Nipponbare 衍生的98个回交重组自交系(BIL)为材料，在缺氧胁迫和正常萌发条件下调查了萌发7 d的幼苗芽鞘长度。以缺氧反应指数为衡量指标，对幼苗耐缺氧能力进行了QTL分析。RIL群体在第2染色体上检测到1个与SSR标记 RM525紧密连锁的QTL qSAT-2-R, 解释表型变异的8.7%；在第7染色体上检测到1个与RM418紧密连锁的QTL qSAT-7-R, 解释表型变异的9.8%；增效等位基因均来自C堡。BIL群体检测到6个QTL,分布在第2、3、5、8、9和12染色体上，分别解释表型变异的16.2%、11.4%、7.3%、5.8%、9.5%和14.0%；其中qSAT-2-B、qSAT-3-B和qSAT-9-B增效等位基因来自Nipponbare。与qSAT-2-B紧密连锁的RFLP标记为C747，C747对应SSR标记RM1367；qSAT-2-R与qSAT-2-B相距7.2 cM。

关键词: 水稻, 幼苗, 耐缺氧能力, 数量性状基因座, 重组自交系, 回交重组自交系

Abstract: Coleoptile lengths(CL) of seedlings at 7 days after germination under anoxic stress and normal conditions were investigated in two permanently segregated populations and their parents in rice (Oryza sativa L.). By using anoxic response index, a ratio of CL under anoxic stress to CL under normal conditions, as indicator of seedling anoxic tolerance (SAT), QTLs for SAT were detected. Two loci controlling SAT, designated as qSAT-2-R and qSAT-7-R, were detected in the RIL population (247 lines) derived from a cross between Xiushui 79(japonica cultivar) and C Bao(japonica restorer line). qSAT-2-R, explaining 8.7% of phenotypic variation, was tightly linked with the SSR marker RM525. qSAT-7-R, explaining 9.8% of phenotypic variation, was tightly linked with the marker RM418. The positive alleles of the two loci came from C Bao. Six loci controlling SAT, designated as qSAT-2-B, qSAT-3-B, qSAT-5-B, qSAT-8-B, qSAT-9-B and qSAT-12-B, were detected in the BIL population (98 lines) derived from a backcross of Nipponbare (japonica) /Kasalath (indica)//Nipponbare(japonica). The positive alleles of qSAT-2-B, qSAT-3-B and qSAT-9-B, which explained 16.2%, 11.4% and 9.5% of phenotypic variation, respectively, came from Nipponbare. The positive alleles qSAT-5-B, qSAT-8-B and qSAT-12-B, which explained 7.3%, 5.8% and 14.0% of phenotypic variation, respectively, were from Kasalath. The RFLP marker C747, linked tightly with qSAT-2-B, corresponded to the SSR marker RM1367 according to physical map. Genetic distance between qSAT-2-R and qSAT-2-B was 7.2 cM.

Key words: rice (Oryza sativa), seedling, anoxic tolerance, quantitative trait locus, recombinant inbred line, backcross recombinant inbred line