中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

耐光氧化水稻体细胞无性系变异体的筛选与初步鉴定

陈以峰 1;孙立华 1;朱永兰 2   

  1. 1 江苏省农业科学院农业生物遗传和生理研究所，南京 210014；2 江苏省练湖农场农业科学研究所，丹阳 212334
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-04-10 发布日期:1997-04-10

Screening and Preliminary identification of Somaclonal Variants Tolerant to Photooxidation in Oryza sativa L.

Chen Yifeng; Sun Lihua; Zhu Yonglan   

  • Received:1900-01-01 Revised:1900-01-01 Online:1997-04-10 Published:1997-04-10

PDF

被引次数

12

摘要： 选用已知耐光氧化的中粳02428与中国91，不耐光氧化的中籼842、酶选明恢63-38、酶选明恢63-53、桂朝2号为供体亲本进行体细胞无性系的培养。从不耐光氧化亲本中可以产生耐与较耐光氧化的无性系变异体，并且突变频率较高，耐光氧化亲本的无性系后代仍然是耐光氧化的，初步观察到耐光氧化变异体具有遗传稳定性。源于中国91的变异体CX1的R5代与R6代孕穗期倒2叶光氧化级数均为Ⅰ级，源于842的变异体842后实的R2代与R3代孕穗期倒2叶光氧化级数均为Ⅱ级。

关键词: 体细胞无性系变异

Abstract: A series of rice somaclonal variants with photooxidation tolerance have been screened and preliminarily identified. It is indicated that somaclonal variants (regenerating plants) from calli of mature caryopsis of 02428 and Chugoku 91 which are tolerable to photooxidation are also tolerant and that those from 842, Meixuan Minghui 63-38, Meixuan Minghui 63-53, and Guichao 2 which are sensitive to photooxidation display a wide spectrum from sensitivity to tolerance under photooxidative condition, among them (168 variants) from 4 sensitive parents 2 variants (numbered as 93012, 93236) have strong ability and 17 variants have medium ability against photooxidation. Decline of net photosynthetic rates of these tolerant variants is remarkably less than that of sensitive variants under photooxidative condition. Moreover it is initially proved in posterities of some variants that this type of variation is of genetic stability. We suggest that rice somaclonal variants with photooxidation tolerance provide a new source of variation which could be used in breeding rice cultivars with higher photosynthetic efficiency in the near future.

Key words: somaclonal variants, photooxidation tolerance, screening