中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

稻种病原菌Acidovorax avenae subsp. avenae的特征化研究

谢关林 1;孙祥良 ２;T.W. MEW ３   

  1. 1 浙江农业大学植物保护系，中国杭州310029；2 浙江省嘉兴市农业科学研究院，中国嘉兴 314001； 3 国际水稻研究所昆虫学和植物病理学部，菲律宾马尼拉933信箱
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-10 发布日期:1998-07-10

Characterization of Acidovorax avenae subsp. avenae from Rice Seeds

Xie Guanlin; Sun Xiangliang; T. W. Mew   

  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-10 Published:1998-07-10

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摘要： 从中国浙江省嘉兴市的稻种上分离出一种细菌，经细菌学、 鉴别培养基、致病性、Biolog及脂肪酸分析测定，表明该细菌属于Acidovorax avenae subsp. avenae。 该细菌在水稻苗期产生褐色条斑，与浙江省60年代报道的细菌性褐条病相似，但在细菌学特征上完全不同。A. avenae subsp. avenae既可从褐变稻谷上，也可从健康种子上分离到。用108和1010个/mL细菌接种稻谷， 种子的发芽及活力明显受到影响。此细菌的种传发病率为0.2%～5.0%，表明种子是传播此种细菌病害的重要侵染源。

关键词: 特征化, Acidovorax avenae subsp. avenae, 种传细菌, 细菌学, 致病性

Abstract: A bacterium was isolated from rice seeds in Jiaxing of Zhejiang Province, China. It has been confirmed as Acidovorax avenae subsp. avenae by bacteriological tests, differential media, pathogenicity test, Biologand fatty acid methyl ester(FAME) analysis(computer aided numeric taxonomic methods). It revealed that A. avenae subsp. avenae is different from the bacterium recorded in Zhejiang in 1960s although they produced similar symptom, bacterial stripe, on rice seedlings. A. avenae subsp. avenae could be isolated from discolored seeds aswell as from healthy looking seeds of rice. They significantly affected seed germination and seed vigor when 1010cfu/mL and 108 cfu/mL of the bacterium were inoculated on rice seeds. The bacterium could transmitted from seeds to seedlings with 0.2%-5.0% of infection. This suggested that rice seed is very infectant infection source fort ransmission of bacterial stripe and care must be taken for introduction of seeds.

Key words: Acidovorax avenae subsp. avenae, bacteriology, characterization, pathogenicity, seedborne bacteria