中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻珍汕97不育胞质与白叶枯病菌早期互作中的活性氧反应

孔繁明 1;许志刚 1;马春红 2;魏建昆 2   

  1. 1 南京农业大学植物保护系， 南京 210095; 2 河北省农业科学院， 石家庄 050051
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-10 发布日期:1998-07-10

Active Oxygen Reaction During Early Stage of Interaction Between Zhenshan 97 Male Sterile Cytoplasm Rice and Xanthomonas oryzae pv. oryzae

Kong Fanming; Xu Zhigang; Ma Chunhong; Wei Jiankun   

  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-10 Published:1998-07-10

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被引次数

32

摘要： 测定了珍汕97不育胞质和正常胞质的稻苗与白叶枯病菌在早期的互作反应，结果表明，互作体系刚建立时，稻叶内立即产生大量的超氧阴离子(O2-)，从而激发超氧物歧化酶(SOD)和脂肪氧合酶(LOX)活性的增高，导致脂质过氧化物丙二醛(MDA)含量的提高。当特异性菌株Ah28接种在不育系材料(亲和组合)上,上述反应的变化幅度和产生量均小于与正常胞质的组合(非亲和组合)，非专化性菌株Os14对活性氧代谢的诱导作用在两种胞质之间差异不明显。这说明不育胞质对白叶枯病较低的抗性和专化性菌株的特异致病性均与较低的活性氧代谢水平有关。

关键词: 不育系, 白叶枯病菌, 超氧阴离子, 超氧物歧化酶, 脂肪氧合酶, 脂质过氧化物

Abstract: Early responses of interaction between two kinds of cytoplasms(male sterile and normal) of Zhenshan 97 and Xanthomonas oryzae pv. oryzae (Xoo) were surveyed. There have been much superoxide anion (O2-) produced in interaction systems during the primary stage of plant-pathogen interaction. Subsequently, superoxide dismutase (SOD) and lipoxygenase (LOX) were stimulated and elevated in activities, and content of lipid peroxide malondialdehyde (MDA) were raised. Induced effect of specific strain Ah28 on active oxygen metabolism (AOM) of Zhenshan 97 male sterile cytoplasm were very different from its normal cytoplasm. The former (compatible system) was remarkably lower than the latter (incompatible system) in metabolic level. However, induced effect of nonspecific strain Os14 had no obvious difference between two kinds of cytoplasms. Results indicated that resistance of male sterile cytoplasm to bacterial leaf blight and distinct pathogenicity of specific strain to rice were related to the lower level of AOM.

Key words: lipid peroxide malondialdehyde, lipoxygenase, male sterile cytoplasm, Xanthomonas oryzae pv.oryzae, superoxide anion, superoxide dismutase