中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

利用RAPD和微卫星标记对协优杂交稻及其亲本进行区别与鉴定

李 莉;杨剑波;汪秀峰;向太和;吴家道   

  1. 安徽省农业科学院 水稻研究所，安徽 合肥 230031
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-10-10 发布日期:2000-10-10

Identification of Xieyou Combinations of Hybrid Rice and Their Three Parents by Using RAPD and Microsatellite Markers

LI Li; YANG Jian-bo; WANG Xiu-feng; XIANG Tai-he; WU Jia-dao   

  1. (Rice Research Institute; Anhui Academy of Agricultural Sciences; Hefei 230031; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-10-10 Published:2000-10-10

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摘要： 选用400个随机寡核苷酸和40个荧光标记的微卫星引物对，对我国主栽的协优杂交稻组合及其亲本进行多态性分析。RAPD分析显示，400个随机寡核苷酸引物中，有364个能够在供试基因型材料中扩增出肉眼可见的DNA条带，其中28个显示扩增的多态性，这中间9个引物产生的13条多态性条带具有清晰、易分辨的特点，可以在供试的水稻组合与亲本之间，以及杂交组合之间进行区别和鉴定。微卫星分析指出，40对荧光标记引物中，13对引物产生的31条标记带，能够在供试基因型材料中显示稳定的多态性，且杂种表现为双亲的互补型。与RAPD相比，微卫星分析具有多态性强、精确性高和重复性好的特点。

关键词: 杂交水稻, 鉴定, 随机扩增多态性DNA, 微卫星

Abstract: Hybrid combinations Xieyou 63, Xieyou 64 and their parents were chosen to carry out RAPD and microsatellite analysis. Four hundred random primers (oligonucleotides) and 40 fluoresent dye labelled microsatellite primer pairs were used for DNA polymorphism test. The results showed that 364 of the 400 random primers amplified visible DNA bands and 28 of these showed evidence of polymorphism, 9 of which were reproduciable and were ultimately selected as criterion primers. All together 13 polymorphic DNA bands from criterion primers were chosen as markers, which could distinguish between or among rice genotypes tested. Microsatellite analysis showed more polymorphism than RAPD, 13 of 40 primer pairs produced polymorphic patterns, all together 31 SSLP markers were gained from 13 primer pairs. For most of primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish different Xieyou F1 hybrids and their parents. By combining both methods, Xieyou combinations of hybrid rice and their three lines can be effectively identified.

Key words: hybrid rice, identification, random amplified polymorphism DNA, microsatellite